Avian and Poultry Biology Reviews 16 (3), 2005

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Cardiac Rhythms in Avian Embryos and Hatchlings

Hiroshi Tazawa

Department of Electrical and Electronic Engineering, Muroran Institute of Technology, Muroran 050-8585, Japan

 

ABSTRACT

Avian embryos develop within the confines of an eggshell independent of maternal physiological functions. The eggshell provides not only an airway for diffusive gas exchange of the embryo, but also a protective barrier between the embryo and the surroundings. The wide space outside the eggshell is available for measurements of embryonic physiological functions. Taking advantage of the hard eggshell, severalmethods have been developed tomeasure cardiogenic signalswhilemaintaining an adequate gas exchange through the eggshell and cardiac rhythms of developing embryos have been determined.This reviewintroduces the measuring methods for cardiogenic signals of embryos and shows daily changes (developmental pattern) of some cardiovascular variables. Daily determinations of heart rate (HR) from the cardiogenic signals exhibit developmental patterns of mean HR (MHR) in embryos of various species of birds. An allometric relationship is found between the fresh egg mass and embryonicMHRin 34 species of birds investigated so far.The developmental patterns ofMHR are also determined by continuous measurements of HR to investigate a presence or absence of infradian, circadian and ultradian rhythms in developing embryos and also hatchlings. For investigation into beat-to-beat rhythms, instantaneous heart rate (IHR) is determined in prepipped and pipped embryos and hatchlings. In chickens, IHR baseline begins to fluctuatewith appearance of transient decelerations with subsequent accelerations during the middle of incubation and distinctive patterns of IHR fluctuations develop in embryos and hatchlings. The patterns and baseline of IHR respond to exposure to altered environments and measurements of their responses have a possibility to elucidate development of some physiological functions.

 

Keywords: embryos, hatchlings, cardiogenic signals, mean heart rate, instantaneous heart rate, developmental pattern, heart rate fluctuation

 

 

Use of the Chicken as an Experimental Animal Model in Atherosclerosis

I. Ayalaa, B. Garcıa Perezb, G. Dome´necha, M.T. Castellsc and M. Valdesb

aDepartment of Veterinary Medicine, College of Veterinary Medicine, University of Murcia, Campus de Espinardo syn, Murcia 30100, SPAIN

bUniversitary Clinical Hospital, Virgen de la Arrixaca. Murcia, Spain

cDepartment of Cell Biology, Medical School, University of Murcia, Spain

 

ABSTRACT

The first evidence of experimental atherosclerosis was reported almost100 years ago. In this review, research into spontaneous and induced atherosclerosis as well as drug intervention studies in the experimental chicken model is discussed together with trends for the future.The chicken is a good animal model for the study of atherosclerosis research, since it presents lipoprotein levels similar to those in humans. It is a biped, and develops spontaneous and induced atherosclerosis mainly in response to high cholesterol diets (being stimulated by hormones and vitamin D), vascular injury or infections (mainlyMarek’s disease) in a similar way to how humans develop atherosclerosis. The chicken atherosclerosis model proved itself useful and very suitable for in vivo drug intervention studies. Emerging risk factors of this potentially fatal disease (such as oxidative stress, inflammation or infection) could be assessed with this model.

 

Keywords: atherosclerosis, chicken, experimental animal model

 

 

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